Article
Complementation of Xanthobacter Py2 mutants defective in epoxyalkane degradation, and expression and nucleotide sequence of the complementing DNA fragment.
Microbiology (Reading, England) - 1 Feb 1995
Swaving J, Weijers C A, van Ooyen A J, de Bont J A
Abstract excerpt
Three Xanthobacter Py2 mutants (M3, M8 and M10) lacking epoxyalkane-degrading activity were isolated and characterized. All mutants were able to grow on acetone, the degradation product of 1,2-epoxypropane conversions. Furthermore, they contained the unidentified 'low molecular mass fraction' (LM...
Topics
- Alkanes
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Epoxy Compounds
- Genetic Complementation Test
- Gram-Negative Aerobic Bacteria
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Open Reading Frames
- Restriction Mapping
- Selection, Genetic
- Sequence Analysis, DNA