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Article

Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120.

Journal of bacteriology - 1 Mar 1991

Buikema W J, Haselkorn R

Abstract excerpt

Approximately 140 mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on media lacking fixed nitrogen (Fix-) were isolated after mutagenesis with diethyl sulfate and penicillin enrichment. A large cosmid library of wild-type Anabaena sp. strain PCC 7120 DNA was constructed in a mini-RK-2 shuttle vector, and seven mutants representing several morphologically abnormal heterocyst phenotypes were...

Topics

Anaerobiosis
Cell Differentiation
Cloning, Molecular
Cosmids
Cyanobacteria
DNA, Fungal
Genes, Bacterial
Genes, Fungal
Genetic Complementation Test
Mutation
Nitrogen Fixation

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Identifiers

PMID: 1900504
DOI: 10.1128/jb.173.6.1879-1885.1991
PMCID: PMC207717

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1900504-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Isolation and complementation of nitrogen fixation mutants of the cyanobacterium Anabaena sp. strain PCC 7120.PMID 1900504Read ↗

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pbpB, a gene coding for a putative penicillin-binding protein, is required for aerobic nitrogen fixation in the cyanobacterium Anabaena sp. strain PCC7120.Semantic proximity Read ↗

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Description of five mutants of the cyanobacterium Anabaena sp strain PCC 7120 affected in heterocyst differentiation and identification of the transposon-tagged genes.Semantic proximity Read ↗

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