Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.

doi:10.1128/aem.59.3.695-700.1993

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Article

Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.

Applied and environmental microbiology - 1 Mar 1993

Muyzer G, de Waal E C, Uitterlinden A G

Abstract excerpt

We describe a new molecular approach to analyzing the genetic diversity of complex microbial populations. This technique is based on the separation of polymerase chain reaction-amplified fragments of genes coding for 16S rRNA, all the same length, by denaturing gradient gel electrophoresis (DGGE). DGGE analysis of different microbial communities demonstrated the presence of up to 10 distinguishable bands in the...

Topics

Base Sequence
Cyanobacteria
DNA, Bacterial
DNA, Ribosomal
Desulfovibrio
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Ethidium
Genes, Bacterial
Genetic Variation
Immunoblotting

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Nucleic Acid Hybridization

Polymerase Chain Reaction

RNA, Bacterial

RNA, Ribosomal, 16S

Sensitivity and Specificity

Thiobacillus

Source

Open in PubMed Open full text

Identifiers

PMID: 7683183
DOI: 10.1128/aem.59.3.695-700.1993
PMCID: PMC202176

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7683183-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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