Article
Construction of a phylogenetic tree for inbred strains of rat by arbitrarily primed polymerase chain reaction (AP-PCR).
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Apr 1995
Canzian F, Ushijima T, Pascale R, Sugimura T, Dragani T A, Nagao M
Abstract excerpt
We constructed a tentative genealogic tree of 13 inbred rat strains using genetic markers identified by the AP-PCR (Arbitrarily Primed Polymerase Chain Reaction) technique, which consists of PCR amplification under low stringency conditions, with only one oligonucleotide as both forward and reverse primers. We obtained discrete-state genotypes of 264 loci, presumably covering the whole rat genome. Computational...
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