Article
Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis.
The American journal of tropical medicine and hygiene - 1 Jun 1995
Morgan U M, Constantine C C, O'Donoghue P, Meloni B P, O'Brien P A, Thompson R C
Abstract excerpt
Genetic variation in 25 Cryptosporidium isolates was analyzed using the random amplified polymorphic DNA (RAPD) technique. Simple reproducible polymorphisms were generated (using five primers) from Cryptosporidium DNA that was free of contaminating bacterial DNA. The results generated by four of...
Topics
- Animals
- Base Sequence
- Cattle
- Cryptosporidium
- DNA Primers
- DNA, Protozoan
- Deer
- Electrophoresis, Agar Gel
- Feces
- Genetic Variation
- Humans
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Reproducibility of Results
- Sheep
- Snakes