Characterization of csh203::Tn917lac, a mutation in Bacillus subtilis that makes the sporulation sigma factor sigma-H essential for normal vegetative growth.

Abstract excerpt

spo0H encodes a sigma factor, sigma-H, of RNA polymerase that is required for sporulation in Bacillus subtilis. Null mutations in spo0H block the initiation of sporulation but have no obvious effect on vegetative growth. We have characterized an insertion mutation, csh203::Tn917lac, that makes spo0H essential for normal growth. In otherwise wild-type cells, the csh203::Tn917lac insertion mutation has no obvious...

Topics

• Bacillus subtilis
• Bacterial Proteins
• Cell Division
• DNA Primase
• DNA-Directed RNA Polymerases
• Gene Expression Regulation, Bacterial
• Mutagenesis, Insertional
• Mutation
• Operon
• Promoter Regions, Genetic