Article
Evaluation of the specificity of alkaline phosphatase-conjugated oligonucleotide probes for forensic DNA analysis.
Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society - 1 Jan 1995
Benzinger E A, Riech A K, Shirley R E, Kucharik K R
Abstract excerpt
Current methods of forensic DNA profiling by restriction fragment length polymorphism analysis rely on radioactive detection of DNA. The use of radioactive isotopes is complicated, expensive and requires elaborate safety precautions. Recently, non-radioactive detection methods involving the use o...
Topics
- Alkaline Phosphatase
- Alleles
- DNA
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Agar Gel
- Forensic Medicine
- Humans
- Luminescent Measurements
- Molecular Weight
- Oligonucleotide Probes
- Phosphorus Radioisotopes
- Polymorphism, Restriction Fragment Length
- Repetitive Sequences, Nucleic Acid
- Sensitivity and Specificity