Detection of T to G mutation at position 59 in the Lewis gene by mismatch polymerase chain reaction.

Abstract excerpt

Recently, we discovered the missense mutations of T to G at position 59 and of G to A at position 508 in one of the Lewis-negative (le) genes (Koda et al. 1993). In the present study, we report a method to detect the mutation at position 59 using mismatch PCR amplification and endonuclease MspI digestion. For this mutation, we found that 7 out of 12 Lewis-negative, and none of 15 Lewis-positive individuals were...

Topics

• Base Sequence
• DNA
• Fucosyltransferases
• Genetic Carrier Screening
• Genome
• Homozygote
• Humans
• Lewis Blood Group Antigens
• Molecular Sequence Data
• Mutation
• Polymerase Chain Reaction