Article
Biophysical factors affecting the expression of isopenicillin N synthase isozymes in Escherichia coli.
Biochemistry and molecular biology international - 1 Apr 1995
Tiow-Suan S, Tan D S
Abstract excerpt
The influence of biophysical factors over the production of isozymes of isopenicillin N synthase (IPNS) from fungal and bacterial sources in Escherichia coli was examined. The effect of these parameters on the expression of Cephalosporium acremonium IPNS was determined by using two expression vectors containing the lac- or the T7-promoter. Under the lac-promoter, IPNS expressed in E. coli reached 5-15% of total...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
