Biophysical factors affecting the expression of isopenicillin N synthase isozymes in Escherichia coli.

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Article

Biophysical factors affecting the expression of isopenicillin N synthase isozymes in Escherichia coli.

Biochemistry and molecular biology international - 1 Apr 1995

Tiow-Suan S, Tan D S

Abstract excerpt

The influence of biophysical factors over the production of isozymes of isopenicillin N synthase (IPNS) from fungal and bacterial sources in Escherichia coli was examined. The effect of these parameters on the expression of Cephalosporium acremonium IPNS was determined by using two expression vectors containing the lac- or the T7-promoter. Under the lac-promoter, IPNS expressed in E. coli reached 5-15% of total...

Topics

Acremonium
Base Sequence
Biophysical Phenomena
Biophysics
Cloning, Molecular
DNA
Escherichia coli
Gene Expression
Genes, Fungal
Genetic Vectors
Isoenzymes
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Oxidoreductases

Proline

Promoter Regions, Genetic

Recombinant Proteins

Solubility

Source

Open in PubMed

Identifiers

PMID: 7549925

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7549925-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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