The cellular concentration of the sigma S subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability.

Abstract excerpt

The second vegetative sigma factor sigma S (encoded by the rpoS gene) is the master regulator in a complex regulatory network that governs the expression of many stationary phase-induced and osmotically regulated genes in Escherichia coli. Using a combination of gene-fusion technology and quantitative immunoblot, pulse-labeling, and immunoprecipitation analyses, we demonstrate here that rpoS/sigma S expression is...

Topics

• Base Sequence
• Cyclic AMP
• DNA Primers
• DNA-Directed RNA Polymerases
• Enzyme Stability
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Gene Expression Regulation, Enzymologic
• Genes, Bacterial
• Genotype