Article
Estimation of diversity and community structure through restriction fragment length polymorphism distribution analysis of bacterial 16S rRNA genes from a microbial mat at an active, hydrothermal vent system, Loihi Seamount, Hawaii.
Applied and environmental microbiology - 1 Mar 1994
Moyer C L, Dobbs F C, Karl D M
Abstract excerpt
PCR was used to amplify (eu)bacterial small-subunit (16S) rRNA genes from total-community genomic DNA. The source of total-community genomic DNA used for this culture-independent analysis was the microbial mats from a deep-sea, hydrothermal vent system, Pele's Vents, located at Loihi Seamount, Hawaii. Oligonucleotides complementary to conserved regions in the 16S rRNA-encoding DNA (rDNA) of bacteria were used to...
Topics
- Bacteria
- Base Sequence
- DNA Fingerprinting
- Genes, Bacterial
- Genetic Variation
- Hawaii
- Molecular Sequence Data
- Polymorphism, Restriction Fragment Length
- RNA, Bacterial
- RNA, Ribosomal, 16S