Article
Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences.
Journal of clinical microbiology - 1 Dec 1993
Hughes M S, Skuce R A, Beck L A, Neill S D
Abstract excerpt
Two methods, based on analysis of the polymerase chain reaction-amplified 16S rRNA gene by restriction enzyme analysis (REA) or direct cycle sequencing, were developed for rapid identification of mycobacteria isolated from animals and were compared to traditional phenotypic typing. BACTEC 7H12 cu...
Topics
- Animals
- Bacterial Typing Techniques
- Bacteriological Techniques
- Base Sequence
- Carnivora
- Cattle
- DNA Restriction Enzymes
- DNA, Bacterial
- Evaluation Studies as Topic
- Genes, Bacterial
- Molecular Sequence Data
- Mycobacterium
- Phenotype
- Polymerase Chain Reaction
- RNA, Bacterial
- RNA, Ribosomal, 16S
- Species Specificity