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Preimplantation diagnosis: Sephadex filtration and human serum albumin gradients do not select spermatozoa by sex chromosome: a fluorescent in-situ hybridization study

1 Oct 1993

Abstract excerpt

Fluorescent in-situ hybridization (FISH) of decondensed sperm nuclei has been used directly to evaluate the enrichment efficiency of human sperm separation using Sephadex gel filtration and human serum albumin (HSA) gradients. Control and processed spermatozoa were fixed and their nuclei decondensed. In-situ hybridization was carried out with a Y-specific DNA probe (DYZ1). Sephadex filtration yielded 52.5%...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 7507937
DOI: 10.1093/oxfordjournals.humrep.a137926

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