Article
Distinct modes of human immunodeficiency virus type 1 proviral latency revealed by superinfection of nonproductively infected cell lines with recombinant luciferase-encoding viruses.
Journal of virology - 1 Feb 1994
Chen B K, Saksela K, Andino R, Baltimore D
Abstract excerpt
To study the basis of cellular latency of human immunodeficiency virus (HIV), we have used a recombinant luciferase-encoding HIV (HXB-Luc) to superinfect nonproductively HIV-1-infected human leukemic cell lines. HXB-Luc contains the Photinus pyralis luciferase gene in place of the nef gene and provides a highly sensitive, simple assay for HIV infection and expression. To circumvent any superinfection block in...
Topics
- Base Sequence
- CD4 Antigens
- Cell Line
- Genes, Reporter
- HIV Reverse Transcriptase
- HIV-1
- Humans
- Luciferases
- Molecular Sequence Data
- Monocytes
- Phenotype