Article
Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory.
Journal of clinical microbiology - 1 Nov 1993
Kirschner P, Springer B, Vogel U, Meier A, Wrede A, Kiekenbeck M, Bange F C, Böttger E C
Abstract excerpt
Clinical isolates of Mycobacterium spp. were identified by direct sequence determination of 16S rRNA gene fragments amplified by polymerase chain reaction. Identification was based on a hypervariable region within the 16S rRNA gene in which mycobacterial species are characterized by species-specific nucleotide sequences. A manually aligned data base including the signature sequences of 52 species of mycobacteria...
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