Article
Comparison of three typing methods for Pseudomonas aeruginosa isolates from patients with cystic fibrosis.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology - 1 Dec 2012
Waters V, Zlosnik J E A, Yau Y C W, Speert D P, Aaron S D, Guttman D S
Abstract excerpt
The aim of this study was to compare two traditional pattern matching techniques, pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD), with the more reproducible technique of multilocus sequence typing (MLST) to genotype a blinded sample of Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients. A blinded sample of 48 well-characterized CF P. aeruginosa isolates was...
Topics
- Cluster Analysis
- Cystic Fibrosis
- DNA Fingerprinting
- Electrophoresis, Gel, Pulsed-Field
- Genotype
- Humans
- Multilocus Sequence Typing
- Predictive Value of Tests
- Pseudomonas Infections
- Pseudomonas aeruginosa
