Article
A genetic analysis of mixed population in Theileria sergenti stocks and isolates using allele-specific polymerase chain reaction.
The Journal of veterinary medical science - 1 Apr 1995
Kubota S, Sugimoto C, Onuma M
Abstract excerpt
In order to differentiate parasite populations bearing two allelic forms of p33/32, an immunodominant piroplasm surface protein of Theileria sergenti, two sets of oligonucleotide primers were designed to amplify either of the two allele by the polymerase chain reaction (PCR). Each set of the primers differentially amplified either of Ikeda- or Chitose-type p33/32 genes. By using this allele-specific PCR and...
Topics
- Alleles
- Animals
- Base Sequence
- Cattle
- Cloning, Molecular
- DNA Primers
- DNA, Protozoan
- Electrophoresis, Agar Gel
- Genes, Protozoan
- Japan
- Molecular Sequence Data