Article
Mutations in conserved domain I of the Sendai virus L polymerase protein uncouple transcription and replication.
Virology - 10 Nov 1995
Chandrika R, Horikami S M, Smallwood S, Moyer S A
Abstract excerpt
To begin to map functional domains of the Sendai P-L RNA polymerase complex we wanted to characterize the P binding site on the Sendai L protein. Analysis of in vitro and in vivo P-L polymerase complex formation with carboxyl-truncations of the L protein showed that the N-terminal half of the protein was required. Site-directed mutagenesis of the Sendai virus L gene was employed to change amino acids within a...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Conserved Sequence
- DNA Primers
- DNA, Viral
- DNA-Directed RNA Polymerases
- Defective Viruses
- Measles virus
- Molecular Sequence Data