Article
Histones of Chlamydomonas reinhardtii. Synthesis, acetylation, and methylation.
Plant physiology - 1 Oct 1995
Waterborg J H, Robertson A J, Tatar D L, Borza C M, Davie J R
Abstract excerpt
Histones of the green alga Chlamydomonas reinhardtii were prepared by a new method and fractionated by reversed-phase high-performance liquid chromatography. Acid-urea-Triton gel analysis and tritiated acetate labeling demonstrated high levels of steady-state acetylation for the single histone H3 protein, in contrast to low levels on histones H4 and H2B. Twenty percent of histone H3 is subject to dynamic...
Topics
- Amino Acid Sequence
- Animals
- Cell Nucleus
- Chlamydomonas reinhardtii
- Chromatography, High Pressure Liquid
- Darkness
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Histones
- Kinetics
- Light
