[Determination of gene mutation of silent serum cholinesterase and its epidemiologic characters in the Japanese].

Abstract excerpt

We detected 121 individuals with silent type of serum cholinesterase from 36 families in Japan. DNA analysis totaling 37 members of eleven blood unrelated families were carried out by four useful methods, namely, 1) PCR-SSCP analysis, 2) dot blot hybridization (DBH) with the use of synthetic oligonucleotide probe, 3) restriction endonuclease analysis (REA) and 4) direct sequencing analysis. Their mutations were...

Topics

• Asian People
• Base Sequence
• Cholinesterases
• DNA
• Female
• Gene Amplification
• Humans
• Japan
• Male
• Molecular Sequence Data
• Mutation
• Pedigree