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Article

Automated laser fluorescence analysis of randomly amplified polymorphic DNA: a rapid method for investigating nosocomial transmission of Acinetobacter baumannii.

Journal of medical microbiology - 1 Dec 1995

Grundmann H, Schneider C, Tichy H V, Simon R, Klare I, Hartung D, Daschner F D

Abstract excerpt

A rapid method for genotyping Acinetobacter baumannii based on PCR-fingerprinting with fluorescent primers was evaluated. Automated laser fluorescence analysis (ALFA) enabled on-line generation of high resolution DNA-fingerprints during polyacrylamide gel electrophoresis of randomly amplified polymorphic DNA (RAPD) products. The results were in concordance with macro-restriction fragment patterns produced by...

Topics

Acinetobacter
Acinetobacter Infections
Base Sequence
Chromosomes, Bacterial
Cross Infection
DNA Fingerprinting
DNA Primers
DNA, Bacterial
Disease Outbreaks
Electrophoresis, Gel, Pulsed-Field

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Electrophoresis, Polyacrylamide Gel

Fluorescence

Fluorescent Dyes

Gene Amplification

Genotype

Germany

Humans

Lasers

Source

Identifiers

PMID: 7473679
DOI: 10.1099/00222615-43-6-446

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7473679-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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