Article
Interaction of hybridoma antibodies with normal and mutant forms of Escherichia coli recA protein.
Biochimie - 1 Jan 2000
Karu A E, Allen L A
Abstract excerpt
We have prepared hybridomas which secrete antibodies against E. coli recA protein, by fusing spleen lymphocytes from immunized mice with P3X63-AG8 myeloma cells. This paper describes a preliminary survey of properties of antibody from a hybridoma population designated 156, which inhibits the strand pairing, strand assimilation, repressor cleavage, and DNA-dependent ATPase activities of recA protein. The 156...
Topics
- Alleles
- Animals
- Antibodies, Monoclonal
- Bacterial Proteins
- Cell Line
- Cross Reactions
- Epitopes
- Escherichia coli
- Genetic Variation
- Hybridomas
- Lymphocytes
- Mice