Article
[Determination of cytochromes in chloroplasts of Chlamydomonas reinhardii mutants].
Prikladnaia biokhimiia i mikrobiologiia - 1 Jan 2000
Roshchina V V, Ladygin V G
Abstract excerpt
A method for qualitative determination of cytochromes of the wild-type and mutant strains of Chalmydomonas reinhardii was developed. The effect of different techniques of cell disruption (ultrasound, Triton X-100, acetone, etc) on detection of cytochrome maxima in the oxidized minus reduced difference spectra was studied on a comparative basis. The development of the stable difference spectrum of the disrupted...
Topics
- Chlamydomonas
- Chloroplasts
- Cytochromes
- Mutation
- Oxidation-Reduction
- Species Specificity
- Spectrophotometry