Article
[Preparation and characteristics of temperature-sensitive mutants of the Venezuelan equine encephalomyelitis virus].
Voprosy virusologii - 1 Jan 2000
Vladyko G V, Tsilinskiĭ Ia Ia
Abstract excerpt
Fifteen ts mutants of Venezuelan equine encephalomyelitis (VEE) virus have been produced and studied. Two of them were isolated from a virus population grown in the presence of 5-fluorouracyl and 13 from a virus suspension treated with nitrous acid. The efficiency of plating 40 degrees/35 degrees of various mutants varied from 2 X 10(-2) to 6 X 10(-6). Three ts mutants had RNA- phenotype (RNA synthesis at a...
Topics
- Encephalitis Virus, Venezuelan Equine
- Fluorouracil
- Mutation
- Nitrous Acid
- Phenotype
- RNA, Viral
- Temperature
- Viral Plaque Assay
