Article
Development of a novel real-time PCR assay to detect intact astA-harboring Escherichia coli in food based on complete DNA sequence of astA gene variants.
International journal of food microbiology - 2 Dec 2026
Arai Sakura, Ooka Tadasuke, Lee Kenichi, Ikeda Nobuyo, Shimmen Kaori, Yokoyama Koji, Arikawa Emi, Kadoguchi Mayumi, Sasaki Mari, Konno Takayuki, Kojima Yuka, Kando Satomi, Konishi Noriko, Kubomura Akiko, Iyoda Sunao, Ohya Kenji, Hirose Shouhei, Ohnishi Takahiro, Hara-Kudo Yukiko
Abstract excerpt
Enteroaggregative Escherichia coli (EAEC) may produce an enterotoxin termed EAEC heat-stable enterotoxin 1 (EAST1), which is encoded by the astA gene. E. coli strains harboring astA but without virulence markers specific for other diarrheagenic E. coli pathotypes (EAST1EC), have been isolated from patients with diarrhea in foodborne outbreaks in Japan. Various nucleotide variants of astA have been reported, some...
Read the complete abstract on PubMedTopics
- Escherichia coli
- Food Microbiology
- Escherichia coli Proteins
- Real-Time Polymerase Chain Reaction