Comparative phenotypic and molecular characterization of primary, sv40-immortalized, and commercial human corneal keratocytes reveals a passage-dependent optimal window for in vitro applications.

Abstract excerpt

BACKGROUND: Keratocyte cells residing in the corneal stroma produce extracellular matrix (ECM) proteins and support transparency. These cells are pivotal for corneal regeneration and the development of in vitro tissue substitutes. However, there is limited literature on the characteristics of primary and immortalized keratocyte cells. METHODS AND RESULTS: This study investigates the preservation of keratocyte...

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• Aldehyde Dehydrogenase
• Cell Line, Transformed
• Collagen Type I
• Cornea
• Corneal Stroma