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Development and clinical validation of a PCR assay for human parvovirus B19 detection with applications in challenging diagnostic scenarios.

Molecular biology reports - 3 Jul 2026

Sharma Kashmi, Shrivastava Rohan, Mishra Somesh, Parihar Poonam, Biswas Debasis, Chaudhary Narendra Kumar, Gohiya Poorva, Jain Mahendra, Nema Ram K, Raut Ashwin A, Gupta Vandana, Pandey Megha Katare

Abstract excerpt

BACKGROUND: Human parvovirus B19 (HB19V) infection poses significant clinical challenges in resource-limited settings, particularly regarding blood safety surveillance and diagnosis of associated syndromes. Limited access to validated diagnostic assays restricts clinical management and transfusion safety protocols. The objective is to develop and validate an in-house duplex real-time PCR assay for HB19V detection...

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Share to DNA, Viral
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Parvoviridae Infections

Share to Parvovirus B19, Human

Share to Real-Time Polymerase Chain Reaction

Share to Reproducibility of Results

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Share to Viral Nonstructural Proteins

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Identifiers

PMID: 42397555
DOI: 10.1007/s11033-026-12266-z

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

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