Mutating alfalfa NAP1 and NAP2 transcription factors by multiplex CRISPR/Cas9 genome editing leads to delayed senescence with improved forage biomass, nutritional quality, and salinity tolerance.

Abstract excerpt

Alfalfa (Medicago sativa L.) is one of the most valuable forage crops due to its high biomass yield potential and nutritional values. However, forage biomass and quality are affected by different environmental and genetic factors, including developmental and stress-induced senescence, management, and harvesting stage. The CRISPR/Cas9 technology targeting stress-responsive transcription factors (TFs) offers great...

Topics

• Medicago sativa
• CRISPR-Cas Systems
• Plant Proteins
• Biomass
• Gene Editing
• Transcription Factors
• Plants, Genetically Modified
• Salt Tolerance
• Plant Senescence