Article
A new SNP genotyping technology Target SNP-seq and its application in genetic analysis of cucumber varieties.
Scientific reports - 27 Mar 2020
Zhang Jian, Yang Jingjing, Zhang Like, Luo Jiang, Zhao Hong, Zhang Jianan, Wen Changlong
Abstract excerpt
To facilitate the utility of SNP-based genotyping, we developed a new method called target SNP-seq which combines the advantages of multiplex PCR amplification and high throughput sequencing. Compared with KASP, Microarrays, GBS and other SNP genotyping methods, target SNP-seq is flexible both in SNPs and samples, yields high accuracy, especially when genotyping genome wide perfect SNPs with high polymorphism and...
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