Telomere-to-telomere genome assembly and a mutant library empower functional genomics and genetic improvement in Cucurbita moschata.

Abstract excerpt

Butternut squash (Cucurbita moschata) is an economically important crop; however, its genetic improvement has been hindered by the lack of high-resolution genomic resources and limited germplasm availability. In this study, we present a gap-free, telomere-to-telomere (T2T) genome assembly of C. moschata PKUMo, generated using high-accuracy Oxford Nanopore reads. The final assembly spans 314.34 Mb and is organized...

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• Centromere
• Cucurbita
• Genome, Plant
• Genomics
• Mutation