Development of a toehold switch detection platform based on a modified cell-free translation system for specific differentiation of coronavirus infectious bronchitis virus genotypes and sensitive detection of phloem-limited bacterium Candidatus Liberibacter asiaticus.

Abstract excerpt

High-resolution and ultrasensitive nucleic acid diagnostic techniques are essential tools for the identification and control of animal and plant pathogens. The toehold switch diagnostic technology based on cell-free systems (CFS) offers rapid response, programmability, versatility, and orthogonality, making it highly promising for addressing animal and plant epidemics. However, the signal amplification...

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