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Article

Artificial Mutations in the Nuclear Gene Encoding Mitochondrial RNA Polymerase Restore Pollen Fertility in Cytoplasmic Male Sterile Tomato.

Plant biotechnology journal - 1 Mar 2026

Kuwabara Kosuke, Nakajima Rika, Gaetan Van Bosstraeten Alexis, Ezura Kentaro, Toriyama Kinya, Ariizumi Tohru, Shirasawa Kenta

Abstract excerpt

Cytoplasmic male sterility (CMS) and restorer of fertility (Rf) are important traits in F1 hybrid breeding. However, the CMS/Rf system has not been used in tomatoes because of the limited resources of Rf lines. In this study, we performed mutagenesis in tomato CMS seeds and successfully obtained 13 suppressor mutants with pollen fertility. Using bulked segregant analysis and whole-genome sequencing for each...

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Identifiers

PMID: 41128168
DOI: 10.1111/pbi.70417
PMCID: PMC12946495

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