Large-scale screening of genes responsible for silique length and seed size in Brassica Napus via pooled CRISPR library.

Abstract excerpt

BACKGROUND: Enhancing rapeseed (Brassica napus, B. napus) yield is critical for ensuring global vegetable oil security. However, yield is heavily influenced by silique development and seed size, the enhancement of which is limited by scarce genetic resources. The CRISPR/Cas9 system has emerged as a powerful tool for constructing genome-wide mutant libraries, even in polyploid crops with complex genomes. RESULTS:...

Topics

• Brassica napus
• Seeds
• CRISPR-Cas Systems
• Gene Editing
• Gene Library
• Genes, Plant
• Phenotype