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Integrating transcriptomics and high-throughput gene editing uncovers shoot apical meristem regulators in Brassica napus.

Plant physiology - 6 Feb 2026

Yu Kaidi, Li Huailin, Hu Yuzhe, Yu Yalun, Deng Songyue, Yang Yang, Guo Mixia, Li Mengting, Zhe Meiling, He Hanzi, Fan Chuchuan

Abstract excerpt

The shoot apical meristem (SAM) determines plant architecture, but the key components of its regulatory network remain elusive in rapeseed (Brassica napus L.). Here, we integrated transcriptomic profiling of 3 multilocular silique mutants (Bnaclv1, Bnaclv2, and Bnaclv3) across key SAM development stages (IM, stage6, and stage8) with large-scale CRISPR/Cas9 functional screening to identify regulators of SAM...

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PMID: 41607132
DOI: 10.1093/plphys/kiag032

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