A telomere-to-telomere Brassica napus genome identifies BnaWRKY44 regulation of seed coat color and oil

Abstract excerpt

Brassica napus yellow-seeded genotypes have higher seed oil content and quality than black-seeded types, but the mechanisms controlling seed coat color (SCC) remain unclear, partly due to limited gap-free references. We generated the telomere-to-telomere genome assembly of the black-seeded cultivar Zhongyou 821 using PacBio HiFi, ultralong ONT, and Hi-C sequencing. Using this reference, genome-wide association...