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Non-standardized protein background in IVF media linked to serum-derived albumin supplementation

13 Sept 2025

Abstract excerpt

PURPOSE: To explore the protein compositional variability of IVF media and identify sources of undeclared contaminants that interfere with the detection of embryo-derived signals. METHODS: Untargeted and targeted mass spectrometry techniques were used to analyze protein composition in 85 samples of used and unused monophasic IVF media across 13 production lots from two manufacturers. Samples included spent...

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PMID: 40944822
DOI: 10.1007/s10815-025-03616-0

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