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Article

Rapid and efficient generation of viral genome knock-in cell lines using the CRISPR-Cas9 system to produce infectious virus.

Journal of virological methods - 1 Dec 2025

Bhatia Pooja, Mohd Aas, Agrawal Ishika, Katiyar Harshita, Goel Amit, Aich Meghali, Chakraborty Debojyoti, Veerapu Naga Suresh

Abstract excerpt

Several medically significant viruses are difficult to propagate with conventional laboratory host systems, limiting their availability for detailed characterization, antiviral screening, and functional studies. A range of methods can be used to generate viruses, such as creating sophisticated cell lines, organoid cultures, and the utilization of animal models. Here, we report the generation and characterization...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 40639438
DOI: 10.1016/j.jviromet.2025.115219

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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