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Article

Constitutively active Arabidopsis cryptochrome 2 alleles identified using yeast selection and deep mutational scanning.

The Journal of biological chemistry - 1 Jun 2025

Taslimi Amir, Jeibmann Axel, Goett-Zink Lukas, Kottke Tilman, Tucker Chandra L

Abstract excerpt

The Arabidopsis blue light photoreceptor cryptochrome 2 (CRY2) responds to blue light to initiate a variety of plant light-based behaviors and has been widely used for optogenetic engineering. Despite these important biological functions, the precise photoactivation mechanism of CRY2 remains incompletely understood. In light, CRY2 undergoes tetramerization and binds to partner proteins, including the...

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Identifiers

PMID: 40409553
DOI: 10.1016/j.jbc.2025.110265
PMCID: PMC12212125

Genes

CRY2
CIB1

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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Constitutively active Arabidopsis cryptochrome 2 alleles identified using yeast selection and deep mutational scanning.PMID 40409553Read ↗

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