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Dual one-step recombinase-aided PCR for rapid detection of Candida in blood

22 Mar 2025

Abstract excerpt

This study presents a novel dual one-step recombinase-aided PCR (DO-RAP) method, combined with recombinant human mannan-binding lectin protein (rhMBL; i.e., M1 protein)-conjugated magnetic bead (M1 bead) enrichment, for the early detection of Candida krusei and Candida parapsilosis bloodstream infections. Unlike previous studies that utilized the characteristic of docosane being solid at room temperature and...

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PMID: 40119195
DOI: 10.1007/s00253-025-13452-y

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