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Establishment of one-step duplex TaqMan real-time PCR for detection of feline coronavirus and panleukopenia virus

14 Feb 2025

Abstract excerpt

A comparative genomic analysis of feline coronavirus (FCoV) and feline panleukopenia virus (FPLV) was performed. Based on the conserved regions of the two viruses, specific probes and real-time PCR (qPCR) primers were designed, and a duplex TaqMan qPCR-based assay was established for detecting FCoV and FPLV. The results showed high analytical specificity, and no cross-response with other feline viruses was...

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PMID: 39951061
DOI: 10.1007/s00253-024-13394-x

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