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Preparing for shotgun sequencing in forensic genetics - Evaluation of DNA extraction and library building methods.

Forensic science international. Genetics - 1 Mar 2025

Kampmann Marie-Louise, Børsting Claus, Jepsen Alberte Honoré, Andersen Mikkel Meyer, Aagreen Clara I V, Poggiali Brando, Jønck Carina Grøntved, Morling Niels, Andersen Jeppe D

Abstract excerpt

Shotgun sequencing can be a powerful tool in forensic genetics, enabling comprehensive genetic analyses of biological samples for human identification (HID), forensic DNA phenotyping, ancestry inference, and forensic investigative genetic genealogy (FIGG). This study evaluated the performance of shotgun sequencing of typical forensic reference samples (whole blood or punches from FTA cards) extracted with four...

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PMID: 39908595
DOI: 10.1016/j.fsigen.2025.103234

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