Article
Comparative Study of Spectral and Functional Properties of Wild Type and Double Mutant H(L173)L/I(L177)H Reaction Centers of the Purple Bacterium Cereibacter sphaeroides.
Biochemistry. Biokhimiia - 1 Oct 2024
Fufina Tatiana Yu, Zabelin Alexey A, Khatypov Ravil A, Khristin Anton M, Shkuropatov Anatoly Ya, Vasilieva Lyudmila G
Abstract excerpt
Previously, we found that in the reaction center (RC) of the purple bacterium Cereibacter sphaeroides, formation of heterodimeric primary electron donor (P) caused by the substitution of His-L173 by Leu, was compensated by the second mutation Ile-L177 - His. Significant changes in the spectral properties, pigment composition, and redox potential of P observed in the H(L173)L RC, are restored to the corresponding...
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