Article
An activity-based sensing fluorogenic probe for monitoring O-methyltransferase in plants.
The New phytologist - 1 Dec 2024
Jia Xin-Lei, Zhu Lixiang, Li Yuanyu, Zhang Pan, Chen Xiao, Shao Kai, Feng Jingxian, Qiu Shi, Geng Jiaran, Yang Yingbo, Wu Zongtai, Xue Jingshi, Wang Ping, Chen Wansheng, Xiao Ying
Abstract excerpt
Activity-based sensing probes are powerful tools for monitoring enzymatic activities in complex biological samples such as cellular and live animals; however, their application in plants remains challenging. Herein, fourteen activity-based fluorescent probes were assayed against Arabidopsis O-methyltransferases (AtOMTs). One probe, 3-BTD, displayed a high selectivity, reactivity, and fluorescence response toward...
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