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Metabolic Labeling and Digital Microfluidic Single-Cell Sequencing for Single Bacterial Genotypic-Phenotypic Analysis.

Small (Weinheim an der Bergstrasse, Germany) - 1 Nov 2024

Guo Junnan, Sun Di, Li Kunjie, Dai Qi, Geng Shichen, Yang Yuanyuan, Mo Mengwu, Zhu Zhi, Shao Chen, Wang Wei, Song Jia, Yang Chaoyong, Zhang Huimin

Abstract excerpt

Accurate assessment of phenotypic and genotypic characteristics of bacteria can facilitate comprehensive cataloguing of all the resistance factors for better understanding of antibiotic resistance. However, current methods primarily focus on individual phenotypic or genotypic profiles across different colonies. Here, a Digital microfluidic-based automated assay for whole-genome sequencing of...

Topics

Single-Cell Analysis
Microfluidics
Genotype
Phenotype
Bacteria
Drug Resistance, Bacterial
Anti-Bacterial Agents

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 39077951
DOI: 10.1002/smll.202402177

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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