Development of real‐time recombinase polymerase amplification ( RPA ) and RPA combined with lateral flow dipstick ( LFD ) assays for the rapid and sensitive detection of cyprinid herpesvirus 3

Abstract excerpt

In this issue, we established rapid, cost-effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA-LFD) and real-time RPA for cyprinid herpesvirus 3(CyHV-3), and evaluated their sensitivity, specificity, and applicability, the real-time RPA method could achieve sensitive diagnosis of CyHV-3 within 1.3 copies per reaction, respectively. The real-time RPA...

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