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Article

Development of a new loop-mediated isothermal amplification test for the sensitive, rapid, and economic detection of different genotypes of Classical swine fever virus.

Frontiers in cellular and infection microbiology - 1 Jan 2024

Bohórquez Jose Alejandro, Muñoz-Aguilera Adriana, Lanka Saraswathi, Coronado Liani, Rosell Rosa, Alberch Mònica, Maddox Carol W, Ganges Llilianne

Abstract excerpt

Background: Classical swine fever virus (CSFV) remains one of the most important pathogens in animal health. Pathogen detection relies on viral RNA extraction followed by RT-qPCR. Novel technologies are required to improve diagnosis at the point of care. Methods: A loop-mediated isothermal amplification (LAMP) PCR technique was developed, with primers designed considering all reported CSFV genotypes. The reaction...

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Identifiers

PMID: 38686097
DOI: 10.3389/fcimb.2024.1372166
PMCID: PMC11056584

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