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PCR combined with lateral flow dipstick assay (PCR-LFD) for a rapid diagnosis of melioidosis

19 Apr 2024

Abstract excerpt

BACKGROUND: Melioidosis is an infectious disease caused by Burkholderia pseudomallei. Septicemic melioidosis patients have a high mortality rate within 48 hours. OBJECTIVE: To develop a polymerase chain reaction (PCR) combined with a lateral flow dipstick (LFD) assay for detection of B. pseudomallei in blood samples. METHODS: The PCR with wcbG gene primers and a PCR-LFD test were developed. The specificity and...

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PMID: 38642327
DOI: 10.12932/ap-021023-1703

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