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Genomic surveillance of SARS-CoV-2 using long-range PCR primers

14 Feb 2024

Abstract excerpt

Introduction: Whole Genome Sequencing (WGS) of the SARS-CoV-2 virus is crucial in the surveillance of the COVID-19 pandemic. Several primer schemes have been developed to sequence nearly all of the ~30,000 nucleotide SARS-CoV-2 genome, using a multiplex PCR approach to amplify cDNA copies of the viral genomic RNA. Midnight primers and ARTIC V4.1 primers are the most popular primer schemes that can amplify...

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PMID: 38440140
DOI: 10.3389/fmicb.2024.1272972

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