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Construction of a Compact Array of Microplasma Jet Devices and Its Application for Random Mutagenesis of Rhodosporidium toruloides.

ACS synthetic biology - 17 Nov 2023

Koh Hyun Gi, Kim Jinhong, Rao Christopher V, Park Sung-Jin, Jin Yong-Su

Abstract excerpt

A small and efficient DNA mutation-inducing machine was constructed with an array of microplasma jet devices (7 × 1) that can be operated at atmospheric pressure for microbial mutagenesis. Using this machine, we report disruption of a plasmid DNA and generation of mutants of an oleaginous yeast Rhodosporidium toruloides. Specifically, a compact-sized microplasma channel (25 × 20 × 2 mm3) capable of generating an...

Topics

Mutagenesis
Mutation
Rhodotorula
DNA

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 37864563
DOI: 10.1021/acssynbio.3c00443

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