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Article

Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis.

PLoS neglected tropical diseases - 1 Oct 2023

Hadipour Mahboubeh, Darani Hossein Yousofi, Talebzadeh Hamid, Eslamian Mohammad, Aboutalebian Shima, Harandi Majid Fasihi, Mirhendi Hossein

Abstract excerpt

BACKGROUND: Developing more sensitive methods for the diagnosis of echinococcosis is essential. In this study PCR assay for sensitive detection of specific cell-free DNA (cfDNA) of Echinococcus granulosus sensu lato in the sera of the sheep naturally infected with echinococcosis was investigated. METHODS: To extract cfDNA from 35 infected sheep, the modified phenol-chloroform method was used for two different...

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PMID: 37856565
DOI: 10.1371/journal.pntd.0011715
PMCID: PMC10617735

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